Taq II

Taq II Source

Purified from E.coli strain that carries the cloned taqRII gene from Thermus aquaticus

Unit Definition

One unit is the amount of enzyme required to digest 1 µg of pBR322 DNA to obtain stable digestion pattern in 1 hour in a total reaction volume of 20 µl

Star Activity

N/A

Reaction Temperature

65°C

Heat Inactivation

No

Assay Unit Substrate

pBR322

Reaction Buffer

50 mM Tris-HCl (pH 8.5 at 25°C)
10 mM MgCl₂
1 mM dithiothreitol

Notes:

1. Avoid multiple cycles of freezing/thawing of the stock reaction buffer no more than 3 times.
2. Thawing should be performed at temperatures not exceeding 10°C.
3. Recommended procedure is to divide the provided reaction buffer into smaller portions and preserve them at -70°C for long-term.
4. Temperature of -20°C should be used only for short-term storage.

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 25°C)
0.1 mM EDTA
200 mM NaCl
1 mM dithiothreitol
200 µg/ml bovine serum albumin
0.02% Triton X-100™
0.02% Tween 20™
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 8.5 at 25°C)
10 mM MgCl₂
1 mM dithiothreitol
1 µg pBR322

Taq II Storage Conditions

Store at -20°C
Shipped on dry ice

Categories

Restriction Endonuleases