Alkaline Phosphatase, Calf Intestinal

Source

Calf intestinal mucosa

Reagents Supplied

10X CIP Reaction Buffer

Applications

• Features higher turnover rate and milder conditions of inactivation than bacterial enzyme (1)
• Can be used to remove 5'-phosphates from DNA or RNA prior to 5'-end labeling (1)
• Used to remove 5'-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures (1)
• Applicable to dephosphorylation of proteins

Unit Definition

One unit is the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenylphosphate to p-nitrophenol in 1 minute at 37°C (2).

Heat Inactivation

No

Concentration

20 - 25 units/μl

Storage Buffer

10 mM Tris-HCl (pH 8.0)
50 mM KCl
1 mM MgCl₂ 
0.1 mM ZnCl₂ 
50% (v/v) Glycerol

Storage Conditions

Store at -20°C
Product shipped on dry ice