RNase I

Description

• Completely nonspecific hydrolyzing after all four bases (1)
• Only available RNase that cleaves the phosphodiester bond of all four bases
• Prefers single-stranded RNA to double-stranded RNA
• Produced from an overexpressing clone in E. coli (2)
• RNase I contains no endonuclease or exonuclease activity toward DNA substrates
• No need for boiling prior to use
• Pure, contains no endonuclease or exonuclease activity toward DNA substrates

Applications

• RNase I degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate
• Ideal for ribonuclease protection assays
• Useful for mapping or quantitation of RNA by selective cleavage of single-strand regions

Reagents Supplied

10X RNase I Reaction Buffer

Unit Definition

One unit is the amount of enzymes required to degrade 1 µg of RNA in 30 minutes at 37°C as detected by TCA precipitation

Storage Buffer

10 mM Tris-HCl (pH 8.0)
200 mM NaCl
50% glycerol

Assay Conditions

10 mM Tris-HCl (pH 8.0)
100 mM NaCl 

Quality Control

All preparations are assayed for contaminating exonuclease and nonspecific endonuclease activities

Storage Conditions

Store at -20°C
Shipped on Dry Ice