Human Cot DNA

Source

Isolated from Human male placenta

Description

• Prepared from human male placenta DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements
• Has been optimized for a size range of 50 to 300 base pairs which represents the theoretical maximum size for repetitive sequences
• 0.5 mg is sufficient for 5 to 10 Southern or 500 in situ hybridizations

Applications

• Decreases false positive signals in Microarrays, in situ Hybridizations, and Southern blots by acting as a blocking agent for repetitive sequences
• Suppresses cross-hybridization to human repetitive DNA  
• Can be used to block non-specific hybridization in microarray screening

Y-Chromosome

Material manufactured from only male placentas

Preparation

Has been prepared by methods including phenol extraction, which is known to inactivate biological agents.
This product should be handled using standard Biosafety Level 2 procedures.

Storage Buffer

10 mM Tris-HCl (pH 7.4)
1.0 mM EDTA 

Quality Control

Determination of Molecular Size: When analyzed on a 2% agarose gel, >50% of Human placenta Cot DNA migrates between 50-300 base pairs.
Comparable Intensity: 3 µg of control lot had intensity in agarose gel with EtBr similar to master lot.
Disease Testing: Materials used in the production of Human Cot DNA tested negative for following diseases (using FDA-approved and licensed methods):

• Hepatitis B Surface Antigen (HBsAg)
• Hepatitis C Antibody Screen (HCV-Ab)
• Syphilis (SYPT or SYPR)
• HIV-1/2 Antibody Screen
• HTLV-I/II Antibody Screen

Note:

CHIMERx takes extraordinary steps to assure that our Human placenta Cot DNA does not exceed theoretical size maximums for repetitive DNA found in the genome of interest, while carefully removing non-Cot fragments which might block a real target site

Storage Conditions

Store at -20°C 
Store the working solution at 4°C 
Shipped on Dry Ice