T4 Gene 32 Protein

Description

• Single-stranded specific DNA binding protein (1)
• Helix destabilizing protein (1)
• Ultrapure recombinant protein

Applications

• Reduces formation of secondary DNA structures
• Prevents degradation of ssDNA by nucleases
• Prevents inhibition of PCR by template NDA contaminants (2)
• Replaces Hot Start method during assembling PCR reaction (4,7)
• Improves the efficiency of DNA amplification by Taq Polymerase (3,4,5,6)
• Enchances the fidelity of modified T4 Polymerase (8)
• Stabilizes single-stranded regions of DNA for site-specific mutagenesis
• Aids completion of restriction enzyme digestion
• Improves the efficiency of DNA synthesis by T4 DNA Polymerase (8)

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 22°C)
100 mM NaCl
1 mM Dithiothreitol
1 mM EDTA
100 mM NaCl
50% (v/v) Glycerol 

Quality Control

All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonulease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

Storage Conditions

Store at -20°C
Shipped on Dry Ice