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T4 DNA Ligase
As low as
$40.00
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
In stock
Only %1 left
SKU
1060
Source
T4 bacteriophage of Escherichia coli
Reagents Supplied
5X T4 DNA Ligase Buffer
Applications
- Catalyzes the joining of duplex DNA molecules at blunt ends (1)
- Covalently joins DNA fragments with complementary cohesive ends
- Seals single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids
- Suitable for cloning of restriction fragments and joining linkers or adapters to blunt-ended DNA (2)
- Ultrapure recombinant enzyme
Unit Definition
One (Weiss) unit is the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-adsorbable form in 20 minutes at 37°C
Note: 67 cohesive end ligation units are the equivalent of one Weiss unit
Heat Inactivation
65ºC for 15 minutes
Storage Buffer
10 mM Tris-HCl (pH 7.5 at 22°C)
50 mM KCl
1 mM Dithiothreitol
50% (v/v) Glycerol
Reaction Conditions
See Certificate of Analysis Below
Storage Conditions
Store at -20°C
Product shipped on dry ice
Certificate of Analysis
Lot 2010013 PDF
MSDS
MSDS PDF
- Weiss, B., Jacquemin-Sablan, A., Live, T.R., Fareed, G.C. and Richardson, C.C. (1968) J. Biol. Chem. 243, 4543-4555
- Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, 1.53-1.73
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