BssH II

Source

Bacillus stearothermophilus H3

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

50°C

Heat Inactivation

80°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM DTT
100 µg/ml bovine serum albumin 

Activity in Buffers

Buffer 1: 100%*
Buffer 2: 100%*
Buffer 3: 100%
Buffer 4: 75%
Buffer 5: 50%
*star activity 

Assay Conditions

See Certificate of Analysis Below

Storage Buffer

10 mM Tris-HCl (pH 7.5)
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
0.02% Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Shipped on dry ice