Hha I

Source

Haemophilus haemolyticus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

No

Heat Inactivation

65°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 μg/ml bovine serum albumin 

Activity in Buffers

Buffer 1: 25%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 75%
Buffer 5: 50%

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 4°C)
100 mM KCl
0.5 mM DTT
500 µg/ml bovine serum albumin
0.1% (w/v) Triton X-100™
50% glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Reaction volume is 50 µl
Incubation is at 37°C for 1 hour

Storage Conditions

Store at -20°C
Shipped on dry ice