Mbo l

Source

Moraxella bovis

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Inactivation Temperature

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Note: Blocked by dam methylation

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 75%
Buffer 5: 75%

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)
0.1 mM EDTA
50 mM KCl
1 mM DTT
0.1% Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

100 µg/ml BSA
10 mM Tris‑HCl (pH 7.5 at 37°C)
50 mM NaCl
10 mM MgCl₂
1 mM dithiothreitol
1 µg Lambda DNA
Incubation is at 37°C for 1 hour in a reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice

Categories

Restriction Endonucleases