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Sac I
As low as
$58.00
5'...GAGCT↓C...3'
3'...C↑TCGAG...5'
In stock
Only %1 left
SKU
2350
Sac I Source
Streptomyces achromogenes
Reagents Supplied
CMX Buffer 4
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Lambda/Hind III DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
CMX Buffer 2;
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin - See more at: https://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin - See more at: https://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf
CMX Buffer 4:
20 mM Tris-Acetate(pH 7.5 at 37°C)
10 mM Magnesium Acetate
50 mM Potassium Acetate
1 mM dithiothreitol
CMX Buffer 2;
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin - See more at: https://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin - See more at: https://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf
Storage Buffer
20 mM Tris‑HCl (pH 8.0 at 4ºC)
150 mM KCl
500 µg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol
Assay Conditions
20 mM Tris-Acetate (pH 7.5 at 37ºC)
10 mM Magnesium Acetate
50 mM Potassium Acetate
1 mM dithiothreitol
1 µg Lambda/Hind III DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
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