Ssp I

Ssp I Source

Sphaerotilus species

Reagents Supplied

CMX Buffer 6

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Star Activity

Yes

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 6
50 mM Tris-HCl (pH 7.4 at Room Temperature)
6 mM MgCl₂
50 mM NaCl
50 mM KCl

Activity in Buffers

Buffer 1: 25%
Buffer 2: 75%
Buffer 3: 50%
Buffer 4: 50%
Buffer 5: 75%
Buffer 6: 100%
*Star activity 

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
500 µg/ml bovine serum albumin
0.2% Triton X-100™
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.4 at Room Temperature)
6 mM MgCl₂
50 mM NaCl
1 µg Lambda DNA
Reaction volume 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases