Taq Single-stranded DNA Binding Protein

Source

Thermus aquaticus 

Description

• Thermostable single-stranded specific DNA binding protein (1)
• Helix destabilizing protein (1)
• Ultrapure recombinant protein

Applications

• Reduces formation of problematic secondary DNA structures
• Prevents degradation of ssDNA by nucleases
• Prevents inhibition of PCR by template DNA contaminants (2)
• Improves the efficiency of DNA amplification by Taq DNA Polymerase (3,4,5,6)
• Improves the specificity and selectivity of multiplex PCR (7)
• Aids PCR of difficult and GC-rich templates
• Stabilizes single-stranded regions of DNA for site-specific mutagenesis
• Aids completion of restriction enzyme digestion

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 22°C)
300 mM NaCl
5 mM β-mercaptoethanol
0.05% Igepal
0.1 mM EDTA
50% (v/v) glycerol

Quality Control

All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

Storage Conditions

Store at -20ºC
Product Shipped on Dry Ice