Vivid Violet™ DNA/RNA Co-Precipitant

Description

• Vivid Violet™ is a neutral carrier which enhances the recovery of nucleic acids when added prior to ethanol precipitation
• Eliminates the need for low incubation temperature
• Functions similarly to RNA or glycogen to improve recovery when precipitating dilute solutions of nucleic acids, but has the advantage of adding no biological material
• Chemically synthesized; will not interfere in subsequent molecular biology manipulations
• Results in a highly visible purple pellet after centrifugation of the precipitation mixture and thus eliminates loss of nucleic acid pellets during ethanol wash steps since the nucleic acids are readily seen
• Avoids the necessity of being removed prior to next molecular biology step
• Helps to determine when nucleic acids are thoroughly dissolved
• Does not interfere with SDA, PCR, RT-PCR, restriction enzyme digestion, gel electrophoresis or fluorometric determination of nucleic acid content
• Does not precipitate nucleic acids shorter than 20 base pairs, unincorporated nucleotides and short primers from PCR products can be removed
• Vivid Violet does absorb at A₂₆₀ and therefore a correction of the absorbance reading is required

Concentration

10.0 mg/ml

Quality Control

All preparations are assayed for contaminating endonuclease, exonuclease, nonspecific RNase and single- and double-stranded DNase activities

Notes

• Sufficient for 100 x 50 μL precipitations
• Supplied at 10 mg/ml
• Stored in nuclease-free water

Procedure

See Certificate of Analysis Below.

Storage Conditions

Store at +4°C
Shipped at room temperature

Categories

Specialty Reagents