Xba I

Source

Xanthomonas badrii

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Lambda/ Hind III DNA fragments in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda/ Hind III DNA fragments

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
1 mM dithiothreitol
50 mM NaCl
100 µg/ml bovine serum albumin
Note: blocked by overlapping Dam methylation

Activity in Buffers

Buffer 1: <25%
Buffer 2: 100%
Buffer 3: 50%
Buffer 4: 100%
Buffer 5: 75%
Buffer 6: 100%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.4 at 4°C)
0.1mM EDTA
1 mM dithiothreitol
500 µg/ml BSA
50% (v/v) glycerol 

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 µg of Lambda/ Hind III DNA
1 mM dithiothreitol
100 µg/ ml bovine serum albumin
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

MSDS PDF- Current Lot

Categories

Restriction Endonucleases